JUDITH KLINMAN

Andrew Banks Family Preeminence Endowed Professor & Department Chair

 

Miami University, B.S. in Systems Analysis
University of Cincinnati, M.S. and Ph.D. in Computer Science

Dr. Klinman received her A.B. and Ph. D. from the University of Pennsylvania in l962 and l966 and then carried out postdoctoral research with Dr. David Samuel at the Weizmann Institute of Science, Israel, and Dr. Irwin Rose at the Institute for Cancer Research, Philadelphia. She was an independent researcher at the Institute for Cancer Research for several years, before moving to the University of California at Berkeley in l978, where she is now a Professor of the Graduate School in the Department of Chemistry, the Department of Molecular and Cell Biology, and the California Institute for Quantitative Biosciences (QB3).

Dr. Klinman has been focused on understanding the fundamental properties that underlie enzyme catalysis. Early in her career, she developed the application of kinetic isotope effects to the study of enzyme catalysis. In l990, she demonstrated the presence of the neurotoxin, 6-hydroxydopa quinone (referred to as TPQ), at the active site of a copper-containing amine oxidase from bovine plasma, overcoming years of incorrect speculation regarding the nature of the active site structure and opening up the field of protein-derived quino-cofactors. Subsequent work from her group showed that the extracellular protein lysyl oxidase, responsible for collagen and elastin cross-linking, contains a lysine cross-linked variant of TPQ, while mechanistic probes advanced knowledge of cofactor biogenesis and catalysis in the copper amine oxidases. Most recent work has unraveled the enigmatic pathway for production of the free-standing bacterial cofactor/vitamin, pyrroloquinoline quinone.

Since the l990s, Klinman’s kinetic studies of enzyme reactions have demonstrated anomalies that demonstrate quantum mechanical hydrogen tunneling in enzyme-catalyzed hydrogen activation reactions. These findings indicate that proteins influence rate by modulating barrier width, not simply barrier height. Because the transfer of hydrogen as a wave requires restructuring of the heavy atom environment, her tunneling studies also provide a direct link between motions within a protein and the bond making/bond breaking processes that they catalyze. Many of the redox enzymes that have been pursued in the Klinman laboratory use molecular oxygen as substrate. She has developed a set of experimental probes for determining the mechanism of oxygen activation. These probes are able to shed light on how proteins can reductively activate O2 to free radical intermediates, while avoiding oxidative damage to themselves. Her most recent investigations are directed at expanding the principles uncovered from hydrogen transfer processes to other classes of enzyme reaction that include the large class of methyltransferases as well as the TIM barrel superfamily. These studies are uncovering a role for evolved protein networks that play a role in thermal transmission from protein-solvent interfaces to the site of catalysis.

get to know Judith

 

What are you most proud of in your work or life?
(1) Changing the way people think about the origins of enzyme catalysis; (2) Breaking the barrier for women in the physical sciences at UCBerkeley

 

What do you like to do when you’re not working?
Read, hike, paint, garden, stay connected with friends and family

 

Do you have an embarrassing moment or moment of failure in your STEM career or academic experience?
My first interview for an academic position (at Bryn Mawr College). Both my husband and postdoc advisor were in the audience and it was an abject disaster!

 

Do you have a personal motto or other words to live by?
Life always looks rosier in the morning after a good night’s sleep


Favorite snack food?

Good days, carrots. Bad days, chocolate cake.

 

What advice would you give your younger self?
Don’t take everything so seriously. Be proud of your accomplishments at each stage.

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Judith Klinman CV

President Barack Obama joins National Medal of Science recipient Judith P. Klinman, University of California, Berkeley, Calif. for a photo in the Blue Room of the White House, Nov. 20, 2014. (Official White House Photo by Lawrence Jackson)